Application
ELISA
Sample
Human Serum (Serum)
Specification
Serum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type
Sandwich
Other product details
Dilution
1/4000
Incubation time
30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
ab27313 was used as the capture antibody in a sandwich ELISA. Plate was coated for 1 hour at room temperature with shaking followed by blocking for 30 minutes in 5% BSA and then antigen exposure for 1 hour. The detection antibody was ab17872, rabbit polyclonal to serum Amyloid P, also at a dilution of 1/4000.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Jennifer Webster
Verified customer
提交于 Aug 16 2006