Application
Western blot
Sample
Human Tissue lysate - whole (Heart, Left Ventricle)
Loading amount
80 µg
Specification
Heart, Left Ventricle
Gel Running Conditions
Reduced Denaturing (10-20% gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/2000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Secondary antibody
Name
Non-Abcam antibody was used: ECL Mouse IgG
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 12 kDa
Additional data
Additional Notes
Gel was run for 60min to avoid running the small sized potein out of the gel.
PVDF membrane was used for transfer, also done for 60min
Lanes 1 and 6 were loaded with Molecular Weight Marker while the rest of the lanes were loaded with left ventricular lysates.
PVDF membrane was used for transfer, also done for 60min
Lanes 1 and 6 were loaded with Molecular Weight Marker while the rest of the lanes were loaded with left ventricular lysates.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dawn Bowles
Verified customer
提交于 Dec 28 2012