Application
Immunohistochemistry (Frozen sections)
Sample
Axolotl Tissue sections (arm 3 days post amputation)
Permeabilization
No
Specification
arm 3 days post amputation
Blocking step
DAKO serum free block as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
none used
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody dilultent reagent solution
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Vector Labs Biotinylated anti-Goat IgG (H+L)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Used DAB staining IMMPRESS
Still seemed to be some background but none on no primary antibody control
Counter stain was hematoxylin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Crystal Jones
Verified customer
提交于 May 24 2018