Application
Western blot
Sample
Human Tissue lysate - whole (stomach)
Loading amount
30 µg
Specification
stomach
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1 % skimmed milk in TTBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Mouse IgG (H+L), Jackson Immunoresearch
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
CCD camera
Exposure
6 minute(s) and 40 second(s)
Bands
Specific: 41 kDa Non-specific: 51 kDa
Additional data
Additional Notes
PDVF mambrane was used.
Figure legend: lane 1 - MW (250, 150, 100, 75, 50, 37, 25, 20, 15, 10), lane 2 - 20 µg, lane 3 - 30 µg, lane 4 - 50 µg of proteins.
Figure legend: lane 1 - MW (250, 150, 100, 75, 50, 37, 25, 20, 15, 10), lane 2 - 20 µg, lane 3 - 30 µg, lane 4 - 50 µg of proteins.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 13 2011