Anti-Sarcomeric Alpha Actinin抗体(ab90776)
Key features and details
- Rabbit polyclonal to Sarcomeric Alpha Actinin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Sarcomeric Alpha Actinin抗体
参阅全部 Sarcomeric Alpha Actinin 一抗 -
描述
兔多克隆抗体to Sarcomeric Alpha Actinin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow -
免疫原
Synthetic peptide corresponding to Human Sarcomeric Alpha Actinin aa 850 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab100828) -
阳性对照
- This antibody gave a positive signal in the following human tissue lysates: Skeletal Muscle; Heart.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab90776于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml.
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa). |
靶标
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功能
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. -
组织特异性
Expressed in both skeletal and cardiac muscle. -
疾病相关
Defects in ACTN2 are the cause of cardiomyopathy dilated type 1AA (CMD1AA) [MIM:612158]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. -
序列相似性
Belongs to the alpha-actinin family.
Contains 1 actin-binding domain.
Contains 2 CH (calponin-homology) domains.
Contains 2 EF-hand domains.
Contains 4 spectrin repeats. -
细胞定位
Cytoplasm > myofibril > sarcomere > Z line. Colocalizes with MYOZ1 and FLNC at the Z-lines of skeletal muscle. - Information by UniProt
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数据库链接
- Entrez Gene: 396263 Chicken
- Entrez Gene: 536607 Cow
- Entrez Gene: 88 Human
- Entrez Gene: 11472 Mouse
- Omim: 102573 Human
- SwissProt: Q3ZC55 Cow
- SwissProt: P35609 Human
- SwissProt: Q9JI91 Mouse
see all -
别名
- Actin binding protein antibody
- Actinin alpha 2 antibody
- ACTN 2 antibody
see all
图片
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All lanes : Anti-Sarcomeric Alpha Actinin antibody (ab90776) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 104 kDa
Observed band size: 104 kDa
Additional bands at: 27 kDa, 30 kDa, 45 kDa, 80 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
IHC image of Sarcomeric Alpha Actinin staining in human Heart FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab90776, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab90776 被引用在 8 文献中.
- Uchimura T et al. A muscle fatigue-like contractile decline was recapitulated using skeletal myotubes from Duchenne muscular dystrophy patient-derived iPSCs. Cell Rep Med 2:100298 (2021). PubMed: 34195678
- Matsui T & Shinozawa T Spontaneous recovery from sunitinib-induced disruption of sarcomere in human iPSC-cardiomyocytes and possible involvement of the Hippo pathway. BMC Pharmacol Toxicol 22:55 (2021). PubMed: 34610839
- Wheelwright M et al. Advancing physiological maturation in human induced pluripotent stem cell-derived cardiac muscle by gene editing an inducible adult troponin isoform switch. Stem Cells N/A:N/A (2020). PubMed: 32497296
- Matsui T et al. Cell-based two-dimensional morphological assessment system to predict cancer drug-induced cardiotoxicity using human induced pluripotent stem cell-derived cardiomyocytes. Toxicol Appl Pharmacol 383:114761 (2019). PubMed: 31533062
- Huang Y et al. Age-Dependent Oxidative DNA Damage Does Not Correlate with Reduced Proliferation of Cardiomyocytes in Humans. PLoS One 12:e0170351 (2017). PubMed: 28099512
- Ma ZG et al. Piperine Attenuates Pathological Cardiac Fibrosis Via PPAR-?/AKT Pathways. EBioMedicine 18:179-187 (2017). PubMed: 28330809
- Gavin KM et al. Hematopoietic-to-mesenchymal transition of adipose tissue macrophages is regulated by integrin ß1 and fabricated fibrin matrices. Adipocyte 6:234-249 (2017). PubMed: 28441086
- Liang P et al. Patient-Specific and Genome-Edited Induced Pluripotent Stem Cell-Derived Cardiomyocytes Elucidate Single-Cell Phenotype of Brugada Syndrome. J Am Coll Cardiol 68:2086-2096 (2016). ICC/IF ; Human . PubMed: 27810048