Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain sections)
Specification
brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Other product details
Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Secondary antibody
Name
Dilution
1/500
Additional data
Additional Notes
I did not obtain any staining with this antibody. I tested it at different dilutions between 1:100-1:3000 using mouse brain sections. This antibody is not good for IHC using perfused-fixed sections.
Abcam response
Many thanks for your feedback, which shows that this antibody does not work in mouse brain sections in IHC-FoFr. This antibody detects both mouse and rat SV2A in Western blot, and your accompanying abreview shows that it detects rat SV2A in IHC-FoFr. It is possible that this antibody does not work in IHC-FoFr in mouse brain sections because the epitope that it recognises adopts a different 3D fold or is not exposed in mouse. We look forward to hearing from other customers who have tried using this antibody in mouse sections in IHC using different methods of antigen retrieval or fixation.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
提交于 Oct 13 2008