Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (SW-620)
Specification
SW-620
Treatment
tranfect siRNA of SUZ12
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS T Milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: DAKO anti rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 75 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
si treated cells
Positive control
WT cells
Additional data
Additional Notes
PyrK had been used ad loading control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 10 2013