Application
Western blot
Sample
Mouse Cell lysate - whole cell (heart tissue lysate)
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Loading amount
40 µg
Specification
heart tissue lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Donkey anti-Rabbit IgG (H + L), 0.1 m
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Odyssey Imaging Systems
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
Positive control
all the samples (SUN2 should be detected in all individual mouse heart lysate )
Additional data
Additional Notes
good antibody specific band the antibody can be reused for several times.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 19 2017