Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (Vascular smooth muscle cell)
Specification
Vascular smooth muscle cell
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 680
Dilution
1/10000
Detection
Detection method
Fluorescence detection
Bands
Specific: 75 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
PubMed ID
YIO415O7-C: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 23 2013