重组Anti-SUN1抗体[EPR6554] (ab124770)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6554] to SUN1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-SUN1抗体[EPR6554]
参阅全部 SUN1 一抗 -
描述
兔单克隆抗体[EPR6554] to SUN1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human SUN1 aa 100-200.
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阳性对照
- IHC-P: Human ovary carcinoma and human breast tissues.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR6554 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab124770于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (4) |
1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
说明 |
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WB
1/1000 - 1/10000. Detects a band of approximately 78-100 kDa (predicted molecular weight: 90 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98°C, below boiling, and then let cool for 10-20 min. |
靶标
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功能
Component of SUN-protein-containing multivariate complexes also called LINC complexes which link the nucleoskeleton and cytoskeleton by providing versatile outer nuclear membrane attachment sites for cytoskeletal filaments. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. Helps to define the distribution of nuclear pore complexes (NPCs). -
序列相似性
Contains 1 SUN domain. -
结构域
The SUN domain may play a role in the nuclear anchoring and/or migration. -
细胞定位
Nucleus inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 23353 Human
- Omim: 607723 Human
- SwissProt: O94901 Human
- Unigene: 438072 Human
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别名
- Protein unc-84 homolog A antibody
- Sad1 and UNC84 domain containing 1 antibody
- Sad1/unc 84 protein like 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling SUN1 with purified ab124770 at 1/200 dilution (0.698 µg/ml). Perform heat mediated antigen retrieval using Citrate buffer, pH 6.0. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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All lanes : Anti-SUN1 antibody [EPR6554] (ab124770) at 1/5000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 90 kDa
Observed band size: 100,90 kDa why is the actual band size different from the predicted?The bands observed are consistent with what has been described in PMID: 16648470
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Unpurified ab124770, at 1/100 dilution, staining SUN1 in paraffin-embedded Human ovary carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab124770 被引用在 12 文献中.
- Janssen A et al. The BAF A12T mutation disrupts lamin A/C interaction, impairing robust repair of nuclear envelope ruptures in Nestor-Guillermo progeria syndrome cells. Nucleic Acids Res 50:9260-9278 (2022). PubMed: 36039758
- Carley E et al. The LINC complex transmits integrin-dependent tension to the nuclear lamina and represses epidermal differentiation. Elife 10:N/A (2021). PubMed: 33779546
- Yang CW et al. Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells. Aging (Albany NY) 13:10490-10516 (2021). PubMed: 33820871
- Ovejero S et al. The Alkylating Agent Methyl Methanesulfonate Triggers Lipid Alterations at the Inner Nuclear Membrane That Are Independent from Its DNA-Damaging Ability. Int J Mol Sci 22:N/A (2021). PubMed: 34299079
- Bikkul MU et al. Telomere elongation through hTERT immortalization leads to chromosome repositioning in control cells and genomic instability in Hutchinson-Gilford progeria syndrome fibroblasts, expressing a novel SUN1 isoform. Genes Chromosomes Cancer 58:341-356 (2019). PubMed: 30474255