Anti-FGE抗体(ab91479)
Key features and details
- Goat polyclonal to FGE
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-FGE抗体 -
描述
山羊多克隆抗体to FGE -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human FGE aa 314-327 (internal sequence).
Sequence:C-ETLNPKGPPSGKDR
Database link: NP_877437.2 -
阳性对照
- Mouse pancreas, Mouse eye and Human kidney lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab91479 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91479于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 40 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Using molecular oxygen and an unidentified reducing agent, oxidizes a cysteine residue in the substrate sulfatase to an active site 3-oxoalanine residue, which is also called C(alpha)-formylglycine. Known substrates include GALNS, ARSA, STS and ARSE. -
组织特异性
Ubiquitous. Highly expressed in kidney, pancreas and liver. Detected at lower levels in leukocytes, lung, placenta, small intestine, skeletal muscle and heart. -
通路
Protein modification; sulfatase oxidation. -
疾病相关
Defects in SUMF1 are the cause of multiple sulfatase deficiency (MSD) [MIM:272200]. MSD is a clinically and biochemically heterogeneous disorder caused by the simultaneous impairment of all sulfatases, due to defective post-translational modification and activation. It combines features of individual sulfatase deficiencies such as metachromatic leukodystrophy, mucopolysaccharidosis, chondrodysplasia punctata, hydrocephalus, ichthyosis, neurologic deterioration and developmental delay. Inheritance is autosomal recessive. -
序列相似性
Belongs to the sulfatase-modifying factor family. -
翻译后修饰
N-glycosylated. Contains high-mannose-type oligosaccharides. -
细胞定位
Endoplasmic reticulum lumen. - Information by UniProt
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数据库链接
- Entrez Gene: 536435 Cow
- Entrez Gene: 484681 Dog
- Entrez Gene: 58911 Mouse
- Entrez Gene: 362409 Rat
- SwissProt: Q0P5L5 Cow
- SwissProt: Q8R0F3 Mouse
- Unigene: 439876 Mouse
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别名
- MGC150436 antibody
- AAPA3037 antibody
- C alpha formylglycine generating enzyme 1 antibody
see all
图片
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Anti-FGE antibody (ab91479) at 1 µg/ml + Mouse Pancreas lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
数据表及文件
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Datasheet download
文献 (0)
ab91479 尚未被引用在任何文献中。