Anti-SCG10抗体(ab21190)
Key features and details
- Goat polyclonal to SCG10
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SCG10抗体
参阅全部 SCG10 一抗 -
描述
山羊多克隆抗体to SCG10 -
宿主
Goat -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human SCG10 aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q93045 -
阳性对照
- Human Brain lysate, A431 lysate ICC/IF: HeLa cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab21190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
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WB |
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa.
A 1 hour primary incubation is recommended for this product. |
说明 |
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ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min). |
WB
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa. A 1 hour primary incubation is recommended for this product. |
靶标
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功能
Is a key regulator of neurite extension through regulation of microtubule instabilily. -
组织特异性
Neuron specific. -
序列相似性
Belongs to the stathmin family. -
翻译后修饰
Sumoylated. -
细胞定位
Cytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms. - Information by UniProt
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数据库链接
- Entrez Gene: 11075 Human
- Omim: 600621 Human
- SwissProt: Q93045 Human
- Unigene: 521651 Human
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别名
- Neuron specific growth associated protein antibody
- Neuronal growth associated protein antibody
- Neuronal growth associated protein (silencer element) antibody
see all
图片
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Ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Anti-SCG10 antibody (ab21190) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).
Predicted band size: 20.8 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab21190 被引用在 2 文献中.
- Davis EJ et al. Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion. Neuroscience 146:784-91 (2007). PubMed: 17331653
- Ohkawa N et al. The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. J Cell Sci 120:1447-56 (2007). PubMed: 17389683