Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TE pH9
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
10% normal buffered formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500

Secondary antibody

Dilution
1/300
Name
Non-Abcam antibody was used: Alexa 647 conjugated donkey anti-rabbit secondary
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
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提交于 Feb 05 2018

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