Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-STAT3 (phospho Y705) antibody [EP2147Y]

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (human esophageal cancer subcutaneous xenograft gro)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
Specification
human esophageal cancer subcutaneous xenograft gro
Blocking step
Non-Abcam blocking agent as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 20°C
Fixative
Formaldehyde

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS+0.1%BSA+0.2%TritonX-100
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: biotinylated anti-rabbit antibody produced in goat
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Username

Tatiana Karakasheva

Verified customer

提交于 Feb 22 2016

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