Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (adult brain Hippocampal region)
Specification
adult brain Hippocampal region
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Diluent: TBS/BSA/Azide
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/300
Additional data
Additional Notes
I compared three anti STAT3 Abcam reagents: this one and also ab15523 and ab31371( see reviews for those).
If I had tested this Ab alone, I would have given it a 5-rating.In adult rat brain there is clear diffuse nuclear staining in scattered nuclei ( these also contain dots of positivity) but most noticably, there is only clear punctate nuclear staining in the vast majority of nuclei ( one or more dots: could these be Nucleolar Organiser Regions?). With the other two Abs I also got nuclear staining but not identical patterns of staining.
I also tested e15 rat embryo and there is a predominance of this puntate nuclear staining in most cells, including non-neuronal cells.
The picture is of a medium -power (x20) area of the rat hippocampal dentate gyrus.
If I had tested this Ab alone, I would have given it a 5-rating.In adult rat brain there is clear diffuse nuclear staining in scattered nuclei ( these also contain dots of positivity) but most noticably, there is only clear punctate nuclear staining in the vast majority of nuclei ( one or more dots: could these be Nucleolar Organiser Regions?). With the other two Abs I also got nuclear staining but not identical patterns of staining.
I also tested e15 rat embryo and there is a predominance of this puntate nuclear staining in most cells, including non-neuronal cells.
The picture is of a medium -power (x20) area of the rat hippocampal dentate gyrus.
Abcam response
We thank the researcher for their honest feedback. We do guarantee this antibody to work for IHC-P in rat and will further investigate its suitability for all rat tissues in paraffin sections.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Feb 29 2008