Western blot abreview for Anti-STAT3 antibody [EPR787Y]

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Spermophilus tridecemlineatus Tissue lysate - whole (brain)
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% BSA in TBS-T
Dilution
1/2000

Secondary antibody

Name
Non-Abcam antibody was used: Immunopure
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
Supersignal West Pico
Bands
Specific: 88, 78, 57, 45, 37 kDa
Exposure
5 minute(s) and 0 second(s)
Positive control
Previous run in ground squirrel brain with P-STAT3 antibody found the same band at ~88 kDa. Verified with a reprobe on the same samples. Also ran a rat brain sample that showed 3 of the bands (88, 57, 37), including the correct one. It is unclear what the identity of the extra bands are.

Additional data

Additional Notes
Lane 1 is rat brain, Lane 2 is ground squirrel brain
Username

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提交于 Apr 06 2015

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