Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-15% TRIS-Glycine SDS PAGE)
Sample
Mouse Tissue lysate - whole (1) mouse embryonic spinal cord E11, 2) HEK293H)
Specification
1) mouse embryonic spinal cord E11, 2) HEK293H
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti rabbit IgG-HRP coupled Jackson Immunores
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Additional data
Abcam response
Many thanks for your valuable feedback. It appears that under these experimental conditions, ab109085 does not specifically detect STAT3. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 17 2014