Application
Immunohistochemistry (Frozen sections)
Blocking step
0.25%TritonX100, 0.2%Gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C
Sample
Mouse Tissue sections (mouse embryonic spinal cord E11)
Specification
mouse embryonic spinal cord E11
Permeabilization
Yes - 0.25%TritonX100
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.25%TritonX100, 0.2%Gelatin
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: donkey anti rabbit Cy3 Jackson Immunoresearch
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3
Dilution
1/400
Additional data
Abcam response
Thank you for your valuable feedback. ab109085 has not been validated for IHC and It appears that under these experimental conditions it does not give specific staining for STAT3 in the mouse embryonic spinal cord. We welcome further customer comments. For more information, please contact scientific support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 28 2014