Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary neurons)
Gel Running Conditions
Reduced Denaturing (10% separating gel)
Loading amount
30 µg
Treatment
IFN-gamma
Specification
Primary neurons
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Skim milk diluted in 1X TBS-T
Dilution
1/500
Secondary antibody
Dilution
1/24000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 790
Detection
Detection method
Fluoroscence
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 87 kDa Non-specific: 52 kDa
Positive control
Cells treated with IFN-gamma
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 21 2017