Application
Immunocytochemistry
Sample
Zebrafish Cultured Cells (embryonic)
Specification
embryonic
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% tritone X 100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 4°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%BSAwith 0.1%tritone X 100
Secondary antibody
Name
Non-Abcam antibody was used: ALEXA FLOUR 488 goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/400
Additional data
Additional Notes
i am unable to deduce results for myself.
Abcam response
We are in contact with this customer in order to provide suggestions for improving the results with this antibody. Indeed other fixations, such as acetone or methanol might could provide better results. With PFA fixation however, it does seem that this antibody does not recongize the Zebrafish SSEA1 protein. Please do let us know if you have any additional information on this subject.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Salma Sana
Verified customer
提交于 Jan 16 2012