Application
Flow Cytometry
Sample
Mouse Cell (mouse ES cell line D3)
Specification
mouse ES cell line D3
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer (gibco) from adherent culture
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation
Paraformaldehyde
Permeabilization
No
Gating Strategy
FSC vs SSC on negative control sample
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: FACS buffer (see above)
Secondary antibody
Name
Non-Abcam antibody was used: PE conjugate goat anti mouse IgM (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Host species: Goat
Clonality: Polyclonal
Conjugation: Phycoerythrin
Dilution
1/200
Additional data
Additional Notes
Exellent antibody for the detection of SSEA1 on mouse ES cells by flow cytometry. Purple profile = negative control. Green profile = SSEA1
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提交于 May 03 2011