Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293H, COS7, mouse hindbrain lysate E14)
Loading amount
30 µg
Specification
HEK293H, COS7, mouse hindbrain lysate E14
Gel Running Conditions
Reduced Denaturing (4-15% SDS gradient gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: goat anti rabbit IgG-HRP coupled
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 150 (GFP tagged 170) kDa Non-specific: 50, 100, 180 kDa
Positive control
HEK293/HeLa cells overexpressing human or mouse srGAP1,2,3, untransfected COS7 (specificity of detection doubtful), embryonic mouse hindbrain full lysate (E14)
Additional data
Additional Notes
seems to react with human and mouse, but high background
Abcam response
Thank you for your valuable feedback. ab75281 has not been validated for cross reactivity with other srGAP family proteins and your data suggests that ab75281 may cross react with srGAP2 and srGAP3.
We hope this information is useful to our customers.
We hope this information is useful to our customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alain Chedotal
Verified customer
提交于 Apr 15 2013