Application
Western blot
Sample
Rat Cell lysate - whole cell (primary rat hepatocytes)
Loading amount
100 µg
Specification
primary rat hepatocytes
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/400
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST + 2.5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
West Pico Chemiluminescent reagent
Exposure
30 second(s)
Bands
Specific: 126 and 60 kDa Non-specific: 80 kDa
Additional data
Additional Notes
Premature and mature SREBP-2 bands were detected, but a non-specific band between 70 and 100 kDa markers was also detected.
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提交于 Feb 18 2011