Anti-SREBP2抗体(ab112046)
Key features and details
- Rabbit polyclonal to SREBP2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SREBP2抗体
参阅全部 SREBP2 一抗 -
描述
兔多克隆抗体to SREBP2 -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Rat, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese hamster, Orangutan -
免疫原
Synthetic peptide corresponding to Human SREBP2 aa 300-350.
Database link: Q12772 -
阳性对照
- HeLa and 293T whole cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
- Epigenetics and Nuclear Signaling
- Transcription
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab112046于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000 - 1/5000. Predicted molecular weight: 124 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 124 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the cholesterol and to a lesser degree the fatty acid synthesis pathway (By similarity). Binds the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') found in the flanking region of the LDRL and HMG-CoA synthase genes. -
组织特异性
Ubiquitously expressed in adult and fetal tissues. -
序列相似性
Belongs to the SREBP family.
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
At low cholesterol the SCAP/SREBP complex is recruited into COPII vesicles for export from the ER. In the Golgi complex SREBPs are cleaved sequentially by site-1 and site-2 protease. The first cleavage by site-1 protease occurs within the luminal loop, the second cleavage by site-2 protease occurs within the first transmembrane domain and releases the transcription factor from the Golgi membrane. Apoptosis triggers cleavage by the cysteine proteases caspase-3 and caspase-7. -
细胞定位
Nucleus and Endoplasmic reticulum membrane. Golgi apparatus membrane. Cytoplasmic vesicle > COPII-coated vesicle membrane. Moves from the endoplasmic reticulum to the Golgi in the absence of sterols. - Information by UniProt
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数据库链接
- Entrez Gene: 395304 Chicken
- Entrez Gene: 100689017 Chinese hamster
- Entrez Gene: 507102 Cow
- Entrez Gene: 6721 Human
- Entrez Gene: 396675 Pig
- Entrez Gene: 300095 Rat
- Omim: 600481 Human
- SwissProt: Q60429 Chinese hamster
see all -
别名
- AI608257 antibody
- bHLHd2 antibody
- Class D basic helix-loop-helix protein 2 antibody
see all
图片
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All lanes : Anti-SREBP2 antibody (ab112046) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 124 kDa
Exposure time: 3 minutes -
Detection of SREBP2 by Western Blot of Immunprecipitate.
Lane 1: ab112046 at 1µg/ml staining SREBP2 in HeLa whole cell lysate immunoprecipitated using ab112046 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab112046 被引用在 5 文献中.
- Zhang D et al. TFCP2 Overcomes Senescence by Cooperating With SREBP2 to Activate Cholesterol Synthesis in Pancreatic Cancer. Front Oncol 11:724437 (2021). PubMed: 34804919
- Fan Z et al. Brahma Related Gene 1 (Brg1) Regulates Cellular Cholesterol Synthesis by Acting as a Co-factor for SREBP2. Front Cell Dev Biol 8:259 (2020). PubMed: 32500071
- Hu Y et al. Regulation effects of total flavonoids in Morus alba L. on hepatic cholesterol disorders in orotic acid induced NAFLD rats. BMC Complement Med Ther 20:257 (2020). PubMed: 32807146
- Li X et al. SREBP-2 promotes stem cell-like properties and metastasis by transcriptional activation of c-Myc in prostate cancer. Oncotarget 7:12869-84 (2016). WB . PubMed: 26883200
- Li X et al. Fatostatin displays high antitumor activity in prostate cancer by blocking SREBP-regulated metabolic pathways and androgen receptor signaling. Mol Cancer Ther 13:855-66 (2014). PubMed: 24493696