Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver)
Loading amount
30 µg
Specification
Liver
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3
Other product details
Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Santa Cruz goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Positive control
Mouse liver, untreated (control) sample
Negative control
mouse heart
Additional data
Additional Notes
The antibody was supposed to detect both precursor (126 kDa) and nuclear (60 kDa) SREBP-1 forms, but no bands were visible. However, Abcam's SREBP-2 antibody worked wonderfully!
Abcam response
Thank you for your feedback.
We were unable to get in contact with the author of this review, but we would suggest blocking for 30 minutes with as little as 0.5% BSA. Also, always make sure that your HRP secondary has not lost activity by checking with a proven antibody.
We were unable to get in contact with the author of this review, but we would suggest blocking for 30 minutes with as little as 0.5% BSA. Also, always make sure that your HRP secondary has not lost activity by checking with a proven antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jun 18 2007