Application
Western blot
Sample
Rat Tissue lysate - whole (Rat liver tissue)
Loading amount
140 µg
Specification
Rat liver tissue
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/400
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2.5% milk in TBS-T(0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit Anti-mouse IgG, HRP-linked Antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
Pierce ECL West Pico chemiluminescent su
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 66 kDa
Additional data
Additional Notes
RIPA buffer was used as the lysis buffer. lane 1- control, lane 2- ovariectomised
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mrs. Smitha Koshy
Verified customer
提交于 Jul 23 2012