Anti-SRD5A1抗体(ab110123)
Key features and details
- Goat polyclonal to SRD5A1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-SRD5A1抗体
参阅全部 SRD5A1 一抗 -
描述
山羊多克隆抗体to SRD5A1 -
宿主
Goat -
经测试应用
适用于: ICC/IF, IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Rat, Human
预测可用于: Mouse, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human SRD5A1 aa 150-250 (internal sequence).
Database link: P18405 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110123于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (3) |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use a concentration of 3.75 µg/ml. ab110123 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin embedded Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with a biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 3.75 µg/ml. ab110123 was validated for use in Immunohistochemistry on a panel of 21 formalin fixed, paraffin embedded Human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with a biotinylated Goat IgG secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. |
靶标
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功能
Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. -
组织特异性
Liver and prostate (at a low level). -
序列相似性
Belongs to the steroid 5-alpha reductase family. -
细胞定位
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 614612 Cow
- Entrez Gene: 403716 Dog
- Entrez Gene: 6715 Human
- Entrez Gene: 78925 Mouse
- Entrez Gene: 24950 Rat
- Omim: 184753 Human
- SwissProt: A5PJS2 Cow
- SwissProt: P18405 Human
see all -
别名
- 3 oxo 5 alpha steroid 4 dehydrogenase 1 antibody
- 3 oxo 5 alpha steroid delta 4 dehydrogenase alpha 1 antibody
- 3-oxo-5 alpha-steroid delta 4-dehydrogenase alpha 1,5-alpha reductase antibody
see all
图片
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Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human small intestine, using ab110123 at 3.75 µg/ml.
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Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human liver, using ab110123 at 3.75 µg/ml.
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Immunohistochemical analyis of SRD5A1 expression in formalin fixed, paraffin embedded Human prostate, using ab110123 at 3.75 µg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab110123 被引用在 7 文献中.
- Hornung RS et al. Sigma-1 receptors and progesterone metabolizing enzymes in nociceptive sensory neurons of the female rat trigeminal ganglia: A neural substrate for the antinociceptive actions of progesterone. Mol Pain 18:17448069211069255 (2022). PubMed: 35040378
- Herrera D et al. Silencing of the transcriptional factor ZEB1 alters the steroidogenic pathway, and increases the concentration of testosterone and DHT in DU145 cells. Oncol Rep 41:1275-1283 (2019). PubMed: 30483800
- Tian H et al. Exposure to cadmium during gestation and lactation affects development and function of Leydig cells in male offspring. Environ Toxicol 33:351-360 (2018). PubMed: 29214744
- Marti N et al. Genes and proteins of the alternative steroid backdoor pathway for dihydrotestosterone synthesis are expressed in the human ovary and seem enhanced in the polycystic ovary syndrome. Mol Cell Endocrinol 441:116-123 (2017). PubMed: 27471004
- Sánchez P et al. Effects of different ethanol-administration regimes on mRNA and protein levels of steroid 5a-reductase isozymes in prefrontal cortex of adolescent male rats. Psychopharmacology (Berl) 231:3273-80 (2014). Rat . PubMed: 24714925
- Castro B et al. Bisphenol A exposure during adulthood alters expression of aromatase and 5a-reductase isozymes in rat prostate. PLoS One 8:e55905 (2013). WB ; Rat . PubMed: 23405234
- Castro B et al. Effects of adult exposure to bisphenol a on genes involved in the physiopathology of rat prefrontal cortex. PLoS One 8:e73584 (2013). WB . PubMed: 24066056