Application
Western blot
Loading amount
60 µg
Gel Running Conditions
Reduced Denaturing (10% PAGE Gel)
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Treatment
100ng/ml IL6 for 15 minutes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 30 minute(s) · Temperature: 4°C · Diluent: 5% non fat milk in TBS with 0.1% Tween20
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 55 kDa
Exposure
30 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 12 2015