Application
Western blot
Sample
Mouse Tissue lysate - whole (brain, liver, RPE)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
5 ug 4-HNE per eye
Specification
brain, liver, RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab from donkey
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
N/A
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 62 kDa Non-specific: 48 kDa
Positive control
oxidant stressed mouse RPE lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 22 2017