Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-12%)
Sample
Human Cell lysate - whole cell (brain gliobastoma from patients)
Specification
brain gliobastoma from patients
Blocking step
No blocking step used for 12 hour(s) and 0 minute(s) · Temperature: 24°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 24°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-IgG secondary antibodies (Li-Cor)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
LI-COR
Bands
Specific: 62 kDa
Exposure
3 minute(s) and 0 second(s)
Additional data
Additional Notes
Our paper is just accepted for Oncotarget Journal
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 13 2015