Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Concentration
1 µg/ml
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 59 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jan 18 2018