Application
Immunocytochemistry
Sample
Human Cell (Hela cells (Epithelial))
Permeabilization
Yes - 0.2% Triron X100
Specification
Hela cells (Epithelial)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Buffer
Dilution
1/2000
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Anti-Rabbit Ig
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Additional data
Additional Notes
The autophagy was inducted by EBSS then lysosomal degradation was inhibited by bafilomycine a1. The figure showing p62 vesicles.
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提交于 Aug 14 2017