Application
Western blot
Sample
Rat Cell lysate - whole cell (rat uterine smooth muscle cells and lung)
Loading amount
50 µg
Specification
rat uterine smooth muscle cells and lung
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS Lait 5%
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Positive control
Lung tissue treated with Triton or not.
The secondary antibody was shown to work fine with other primary antibodies.
The secondary antibody was shown to work fine with other primary antibodies.
Negative control
Transfected cells using Spns2 siRNA
Additional data
Abcam response
This antibody seems to be unsuitable for Rat.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Zahra Tanfin
Verified customer
提交于 Jul 06 2011