Application
Western blot
Sample
Human Cell lysate - nuclear (seminoma)
Gel Running Conditions
Reduced Denaturing (Bolt Bis-Tris 4-12% SDS PAGE)
Loading amount
156000 cells
Specification
seminoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA / 0.01% TBS Tween
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: LiCOR Donkey-anti-mouse 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
LiCOR CLx
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 44 kDa
Positive control
Sample is Sox17+
Additional data
Additional Notes
VERY SPECIFIC single band! Overlapping band with another specific Sox17 antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Apr 10 2017