Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/250
Additional data
Additional Notes
Submitted image shows excellent immunolabelling of nuclei of cells in the svz and exit zone of the Lateral ventricle of adult rat.
In other areas of this brain, there are isolated groups of cells also with positive nuclei ( not shown)
I am not yet familiar with the function/distribution pattern of this protein thus, cannot explain this.
In other areas of this brain, there are isolated groups of cells also with positive nuclei ( not shown)
I am not yet familiar with the function/distribution pattern of this protein thus, cannot explain this.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Sep 21 2012