Application
IHC - Wholemount
Sample
Mouse Embryo (E8.5)
Specification
E8.5
Other product details
Incubation time
48 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% Donkey serum/1%BSA/PBST
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 647 Donkey Anti-Rabbit IgG(H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Fixed with 4% PFA at 4C for 3hrs, permeabilised with 0.1% PBST and neutralised with 1M Glycine/PBST pH7-8. No nuclear staining observed.
Abcam response
Thank you for sharing this data with us. ab109290 has not previously been tested in IHC wholemount on mouse embryos. However, based on the data shown here, ab109290 does not work in this application, under these experimental conditions. We would welcome feedback from other researchers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Dec 08 2016