Application
Western blot
Sample
Silk worm Tissue lysate - whole (testis lysate (silkworm), BmN4 cell lysate (silkwo)
Gel Running Conditions
Non-reduced Denaturing (12% polyacrylamide)
Loading amount
10 µg
Specification
testis lysate (silkworm), BmN4 cell lysate (silkwo
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk / PBS-T
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
recombinant silkworm SOD1
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 24.2 kDa
Positive control
10µg HeLa cell lysate
Additional data
Additional Notes
lane1 10µg HeLa cell, lane2 BmN4 cell lysate, lane3 10µg silkworm testis lysate, lane4 recombinant silkworm SOD1, lane5 recombinant silkworm SOD2.
PubMed ID
25714339: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
陽水 野島
Verified customer
提交于 Aug 17 2016