Anti-SOCS3抗体[SO1] (ab14939)
Key features and details
- Mouse monoclonal [SO1] to SOCS3
- Suitable for: Flow Cyt, ELISA, WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG2b
概述
-
产品名称
Anti-SOCS3抗体[SO1]
参阅全部 SOCS3 一抗 -
描述
小鼠单克隆抗体[SO1] to SOCS3 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, ELISA, WB, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Full length native protein (purified) (Human).
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SO1 -
同种型
IgG2b -
研究领域
相关产品
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14939于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ELISA |
Use at an assay dependent concentration.
|
|
WB | (2) |
Use at an assay dependent concentration.
|
ICC/IF |
Use a concentration of 5 µg/ml.
|
说明 |
---|
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
靶标
-
功能
SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST. -
组织特异性
Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus. -
通路
Protein modification; protein ubiquitination. -
疾病相关
Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients. -
序列相似性
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
结构域
The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. -
翻译后修饰
Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1. - Information by UniProt
-
数据库链接
- Entrez Gene: 9021 Human
- Entrez Gene: 89829 Rat
- Omim: 604176 Human
- SwissProt: O14543 Human
- SwissProt: O88583 Rat
- Unigene: 527973 Human
- Unigene: 127801 Rat
-
别名
- ATOD4 antibody
- CIS 3 antibody
- CIS-3 antibody
see all
图片
-
ICC/IF image of ab14939 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14939, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Overlay histogram showing Jurkat cells stained with ab14939 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14939, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (12)
ab14939 被引用在 12 文献中.
- Li M et al. Axl is related to inflammation in hemodialysis patients. Mol Immunol 133:146-153 (2021). PubMed: 33667984
- Liang E et al. COP9 Signalosome Subunit 3 Restricts Neuroinflammatory Responses During Cerebral Ischemia/Reperfusion Injury Through Stabilizing Suppressor of Cytokine Signaling 3 Protein. Neuropsychiatr Dis Treat 17:1217-1227 (2021). PubMed: 33911869
- Luo Y et al. Inhibition of EZH2 (Enhancer of Zeste Homolog 2) Attenuates Neuroinflammation via H3k27me3/SOCS3/TRAF6/NF-?B (Trimethylation of Histone 3 Lysine 27/Suppressor of Cytokine Signaling 3/Tumor Necrosis Factor Receptor Family 6/Nuclear Factor-?B) in a Rat Model of Subarachnoid Hemorrhage. Stroke 51:3320-3331 (2020). PubMed: 32933418
- Huang K et al. Long noncoding RNA MALAT1 promotes high glucose-induced inflammation and apoptosis of vascular endothelial cells by regulating miR-361-3p/SOCS3 axis. Int J Clin Exp Pathol 13:1243-1252 (2020). PubMed: 32509100
- Mak HK et al. MicroRNA-19a-PTEN Axis Is Involved in the Developmental Decline of Axon Regenerative Capacity in Retinal Ganglion Cells. Mol Ther Nucleic Acids 21:251-263 (2020). PubMed: 32599451