Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (12% SDS-PAGE)
Sample
Human Cell lysate - nuclear (HeLa)
Specification
HeLa
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 18°C · Diluent: PBS tween 5% milk
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL prime
Bands
Specific: 130 kDa Non-specific: 20 kDa
Exposure
1 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 05 2013