Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse liver cell line)
Loading amount
15 µg
Specification
mouse liver cell line
Gel Running Conditions
Reduced Denaturing (8%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: fluorescence (680) labeled antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Dilution
1/10000
Detection
Detection method
Fluorescence
Exposure
1 hour(s), 0 minute(s) and 0 second(s)
Additional data
Additional Notes
No specific bands observed under described conditions.
May be functional in other cell lines or applications.
Picture taken with Odessey Fluorscence Scanner (LiCor).
Scan intensity 7
May be functional in other cell lines or applications.
Picture taken with Odessey Fluorscence Scanner (LiCor).
Scan intensity 7
Abcam response
Thank you for the valuable feedback. It may be necessary to increase the concentration of primary antibody to see the specific signal. Also, use of a positive control, such as the SH-SY 5Y cell lysate, would be optimal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Frank Götschel
Verified customer
提交于 Sep 09 2009