Application
Western blot
Sample
Mouse Cell lysate - whole cell (Oocytes)
Loading amount
100 cells
Specification
Oocytes
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: HRP goat anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 110 kDa Non-specific: 90 kDa
Positive control
Liver extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 30 2007