Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat E17 cortical neurons 7DIV)
Specification
Rat E17 cortical neurons 7DIV
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% triton & 3% BSA for 30 minutes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 3% BSA in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/300
Additional data
Additional Notes
Image legend:
Blue is Dapi, green is anti-MAP2 (1:500) and red is anti-Brm (1:100).
Blue is Dapi, green is anti-MAP2 (1:500) and red is anti-Brm (1:100).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 22 2010