Anti-SMAD9抗体(ab48011)
Key features and details
- Goat polyclonal to SMAD9
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SMAD9抗体
参阅全部 SMAD9 一抗 -
描述
山羊多克隆抗体to SMAD9 -
宿主
Goat -
特异性
Not expected to crossreact with SMAD1 or SMAD5. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Chicken, Dog -
免疫原
Synthetic peptide:
C-HSEPLMPHNATYPD
, corresponding to internal sequence amino acids 166-179 of Human SMAD9. -
阳性对照
- Human skin lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.05% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab48011于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD). -
组织特异性
Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney. -
疾病相关
Pulmonary hypertension, primary, 2 -
序列相似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻译后修饰
Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase. -
细胞定位
Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4. - Information by UniProt
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数据库链接
- Entrez Gene: 4093 Human
- Entrez Gene: 55994 Mouse
- Entrez Gene: 85435 Rat
- Omim: 603295 Human
- SwissProt: O15198 Human
- SwissProt: Q9JIW5 Mouse
- SwissProt: O54835 Rat
- Unigene: 123119 Human
see all -
别名
- MAD homolog 9 antibody
- Madh6 antibody
- Mothers against decapentaplegic antibody
see all
图片
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Anti-SMAD9 antibody (ab48011) at 0.3 µg/ml + Human Skin lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (1)
ab48011 被引用在 1 文献中.
- Katakawa Y et al. Smad8/9 Is Regulated Through the BMP Pathway. J Cell Biochem 117:1788-96 (2016). PubMed: 26748560