Application
Western blot
Sample
Human Cell lysate - whole cell (Human prostate and cervical cancer cell)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
30 µg
Specification
Human prostate and cervical cancer cell
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBST buffer
Dilution
1/500
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Not included
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 52 kDa
Positive control
Hela Cell with TGF beta treatment
Additional data
Additional Notes
This time I showed the marker of the membrane. Previously I didn't show it cause I think the bands are enough to show where the p-smad5 is. Please check if the membrane is OK or not. Thanks.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 20 2015