Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse aortic smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
25 µg
Treatment
Cre or GFP adenovirus 6 hrs
Specification
Mouse aortic smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Goat anti-rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 26 kDa
Positive control
My samples
Additional data
PubMed ID
27499244: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 15 2018