Application
Western blot
Sample
Human Cell lysate - whole cell (HUVEC cell lysate)
Gel Running Conditions
Reduced Denaturing (11% SDS-PAGE)
Loading amount
30 µg
Treatment
5ng/ml TNF-alpha for 24hrs
Specification
HUVEC cell lysate
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in TBST
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL pico
Negative control
Non-treated HUVEC cells
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 23 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 11 2013