Application
Immunohistochemistry (Frozen sections)
Sample
Chicken Cell (Embryonic section of spinal cord, stage 10-11)
Specification
Embryonic section of spinal cord, stage 10-11
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1%
Other product details
Dilution
1/500
Incubation time
14 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Donkey anti-Rabbit
Host species: Donkey
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Donkey
Clonality: Monoclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Fixation was in 4% PFA in phosphate buffer at 4oC for 2h.
Blocking and antibody steps were carried out in 1% BSA in PBS with 0.1% Triton.
Blocking and antibody steps were carried out in 1% BSA in PBS with 0.1% Triton.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. James Briscoe
Verified customer
提交于 Nov 10 2008