Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 breast cancer)
Loading amount
10 µg
Specification
MCF7 breast cancer
Treatment
3 of the samples treated with Snail, Slug or Control adenovirus for 2 days
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + 0.1% Tween 20 + 5% Milk
Secondary antibody
Name
Non-Abcam antibody was used: GE Amersham
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Prime
Exposure
30 second(s)
Bands
Specific: ~30 kDa
Positive control
MCF-7 infected with Slug-adenovirus (Lane1)
Negative control
MCF-7 cell lysate (Lane 4)
Additional data
Additional Notes
This is an excellent Slug antibody, it does not cross-react with Snail, like many of them do.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 18 2012