Anti-SLC25A13/Citrin抗体(ab96303)
Key features and details
- Rabbit polyclonal to SLC25A13/Citrin
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SLC25A13/Citrin抗体
参阅全部 SLC25A13/Citrin 一抗 -
描述
兔多克隆抗体to SLC25A13/Citrin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Zebrafish -
免疫原
Recombinant fragment corresponding to Human SLC25A13/Citrin aa 248-505.
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阳性对照
- IHC-P: Mouse kidney tissue;N87 xenograft tissue. ICC/IF: HeLa cells. WB: Mouse liver lysate; rat liver lysate; HepG2, HEK-293T, A431 and HeLa whole cell extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96303于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 74 kDa.
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说明 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 74 kDa. |
靶标
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功能
Catalyzes the calcium-dependent exchange of cytoplasmic glutamate with mitochondrial aspartate across the mitochondrial inner membrane. May have a function in the urea cycle. -
组织特异性
High levels in liver and low levels in kidney, pancreas, placenta, heart and brain. -
疾病相关
Defects in SLC25A13 are the cause of citrullinemia type 2 (CTLN2) [MIM:603471]. Citrullinemia belongs to the urea cycle disorders. It is an autosomal recessive disease characterized primarily by elevated serum and urine citrulline levels. Ammonia intoxication is another manifestation. CTLN2 is characterized by neuropsychiatric symptoms including abnormal behaviors, loss of memory, seizures and coma. Death can result from brain edema. Onset is sudden and usually between the ages of 20 and 50 years.
Defects in SLC25A13 are the cause of neonatal intrahepatic cholestasis due to citrin deficiency (NICCD) [MIM:605814]. NICCD is a form of citrullinemia type 2 with neonatal onset. NICCD is characterized by suppression of the bile flow, hepatic fibrosis, low birth weight, growth retardation, hypoproteinemia, variable liver dysfunction. NICCD is generally not severe and symptoms disappear by one year of age with an appropriate diet. Years or even decades later, however, some individuals develop the characteristic features of citrullinemia type 2 with neuropsychiatric symptoms. -
序列相似性
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
细胞定位
Mitochondrion inner membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 615470 Cow
- Entrez Gene: 10165 Human
- Entrez Gene: 50799 Mouse
- Entrez Gene: 362322 Rat
- Omim: 603859 Human
- SwissProt: Q9UJS0 Human
- SwissProt: Q9QXX4 Mouse
- Unigene: 489190 Human
see all -
别名
- AI785475 antibody
- ARALAR2 antibody
- Calcium binding mitochondrial carrier protein Aralar2 antibody
see all
图片
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4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling SLC25A13/Citrin using ab96303 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
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All lanes : Anti-SLC25A13/Citrin antibody (ab96303) at 1/3000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa -
Paraffin-embedded mouse kidney tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: Citrate buffer, pH 6.0, 15 min.
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Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + Mouse liver lysate at 50 µg
Predicted band size: 74 kDa -
Paraffin-embedded N87 xenograft tissue labeling SLC25A13/Citrin using ab96303 at 1/500 dilution in immunohistochemical analysis.
Antigen retrieval: EWDTA-based (pH 8.0), 15 minutes.
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Anti-SLC25A13/Citrin antibody (ab96303) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Predicted band size: 74 kDa -
Anti-SLC25A13/Citrin antibody (ab96303) at 1/500 dilution + Rat liver lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 74 kDa
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab96303 被引用在 1 文献中.
- Koshenov Z et al. Citrin mediated metabolic rewiring in response to altered basal subcellular Ca2+ homeostasis. Commun Biol 5:76 (2022). PubMed: 35058562