Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Rat Tissue lysate - whole (aorta)
Specification
aorta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Bands
Specific: 110 kDa Non-specific: 38 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
The SIRT1 bands are very clear. There is no non-specific band.
Abcam response
Thank you for your review. Although our data has previously shown the antibody does not react, this review indicates it may possibly work in certain samples. We appreciate the feedback!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 22 2013